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Study on Dissociation Equilibria of Eberconazole Nitrate in Micellar Media by Spectrophotometry
Marothu Vamsi Krishna,
Salah Ali Mahgoub Idris,
G. Madhavi,
B. Jalachandra Reddy,
M. Sowhardhra,
D. Gowri Sankar
Issue:
Volume 5, Issue 5, September 2019
Pages:
48-51
Received:
15 August 2019
Accepted:
9 September 2019
Published:
11 October 2019
Abstract: The equilibrium dissociation constant (KD) is the basic parameter to evaluate the binding property of the chemical structure of compounds. Thus, a variety of analytical methods have been established to determine the KD values, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affinity chromatography, and isothermal titration calorimetry. Here we present a detailed overview of the dissociation equilibria of Eberconazole nitrate (EBZ) in homogeneous and heterogeneous systems, focusing primarily on methods that are based on spectrophotometrically of the dissociation reaction. The Dissociation equilibria of Eberconazole nitrate (EBZ) in homogeneous and heterogeneous systems were studied spectrophotometrically in Britton-Robinson’s (BR) buffer at 25°C. Acidity constant of EBZ in BR buffer was found to be 9.5. The effect of anionic, cationic and non-ionic surfactants applied in the concentration exceeding critical micellar concentration (cmc) on acid – base properties of EBZ were also examined. The results revealed a shift of pKa values in micellar media comparing to the values obtained in BR buffer. These shifts in pKa values are more in cationic and anionic micellar media compared with that of non-ionic. The observed differences in pKa values between micellar media and BR buffer solution ranged between -6.0 to -2.0 units. The micellar-mediated pKa shifts can be attributed to the differences between the mean intrinsic solvent properties of the interfacial and bulk phases, with an additional contribution from the electrostatic micellar surface potential in the case of the charged aqueous micellar solutions.
Abstract: The equilibrium dissociation constant (KD) is the basic parameter to evaluate the binding property of the chemical structure of compounds. Thus, a variety of analytical methods have been established to determine the KD values, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affin...
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Physico-chemical Quality Assessment of Palm Kernel Oil During Storage at Room Temperature and Shelter of Light
Gadegbe Afiwa Viviane,
Bakoma Batomayena,
Novidzro Kosi Mawuéna,
Dotse Kokouvi,
Melila Mamatchi
Issue:
Volume 5, Issue 5, September 2019
Pages:
52-60
Received:
25 September 2019
Accepted:
11 October 2019
Published:
21 October 2019
Abstract: Vegetable oils, including palm kernel oil, are an important part of the human diet. However, traditional methods of storing them often do not take into account changes in quality. In addition, these are food products whose rancidity by auto-oxidation could affect the health of the consumer. The objective of this work is to evaluate the physico-chemical quality of palm kernel oil during storage at room temperature and away from light. The palm kernel oil samples were collected in plastic containers under strict hygienic conditions, wrapped in aluminium foil and stored at ambient temperature away from light. Physico-chemical parameters were determined for fresh samples and for samples stored after 180 days. The results showed that after 180 days, the oils analysed showed the onset of oxidation. However, the characteristics after 180 days of storage indicate that the oils still have a relatively acceptable quality compared to Codex Alimentarius standards, concomitantly with the high content of saturated fatty acids. Thus, away from light, palm kernel oils could be stored for six months at ambient temperature with a lower risk of rancidity. However, the relative deterioration observed after 180 days of storage requires precautions in the conservation of palm kernel oils produced in Togo.
Abstract: Vegetable oils, including palm kernel oil, are an important part of the human diet. However, traditional methods of storing them often do not take into account changes in quality. In addition, these are food products whose rancidity by auto-oxidation could affect the health of the consumer. The objective of this work is to evaluate the physico-chem...
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Microscopic Studies, Phytochemical and Biological Screenings of Ocimum canum
Dorothée Dinangayi Tshilanda,
Clément Liyongo Inkoto,
Kashala Mpongu,
Souzane Mata,
Paulin Kapepula Mutwale,
Damien Sha-Tshibey Tshibangu,
Gédéon Ngiala Bongo,
Ngbolua Koto-te-Nyiwa,
Pius Tshimankinda Mpiana
Issue:
Volume 5, Issue 5, September 2019
Pages:
61-67
Received:
15 August 2019
Accepted:
7 October 2019
Published:
25 October 2019
Abstract: It has been reported that more than 80% of the African population uses traditional medicine to primary healthcare. The aim of the study was to determine the histological elements, the phytochemical composition and evaluate the bioactivities of Ocimum canum in order to promote this plant used in Congolese traditional medicine. The earthworms were collected from the ponds of the secondary forest of Monastère Prieure Notre Dame de l’Assomption in Kinshasa city and the blood used in this study was provided by the Centre de Médecine Mixte et d’Anémie SS in Yolo-Sud quarter, Kinshasa, Democratic Republic of the Congo. The histological elements were determined by microscopic examination while different metabolites were determined using thin layer chromatography. Microscopic examination revealed the presence of crystalliferous fibres, fragment of parenchyma, grandular and non-glandular pluricellular trichomes, spheroidal pollen grains, diacytic stomata, spiral vessels. With regard to chromatographic analysis, phytochemicals identified were anthocyanins, coumarins, flavonoids, terpenoids and iridoids. The extracts from O. canum showed good antihelminthic, antisickling and antioxidant activities related to their phytochemicalsAll these findings constitute a scientific evidence validating the use of this medicinal plant for the management of various ailments in the Democratic Republic of the Congo.
Abstract: It has been reported that more than 80% of the African population uses traditional medicine to primary healthcare. The aim of the study was to determine the histological elements, the phytochemical composition and evaluate the bioactivities of Ocimum canum in order to promote this plant used in Congolese traditional medicine. The earthworms were co...
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